Coiled Coil Domain Containing 142 - Alchetron, the free social encyclopedia

6). An enzyme isolated from a liver cell is run on a gel filtration column. The protein elutes at arate suggesting it has a molecular mass of 250,000 Daltons. The isolated protein is run in an SDS-PAGE gel in the absence of β-mercaptoethanol or dithiothreitol (no reducing agent), and appearsas a single band at 50,000 Daltons. A repeat of the SDS-PAGE gel in the presence of β-mercaptoethanol show the protein resolving into two separate bands: one band at 20,000 Daltonsand one band at 30,000 Dalto